High-yield production of major T-cell ESAT6-CFP10 fusion antigen of M. tuberculosis complex employing codon-optimized synthetic gene
نویسندگان
چکیده
منابع مشابه
Oral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10
Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data...
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متن کاملEffects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages
BACKGROUND Mycobacterium tuberculosis is the major pathogen of tuberculosis, which affects approximately one-third of the world's population. The 6 kDa early secreted antigenic target (ESAT6) and the 10 kDa culture filtrate protein (CFP10), which are secreted by the ESX-1 system in M. tuberculosis, can contribute to mycobacterial virulence. OBJECTIVES The aim of this study was to research the...
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متن کاملeffects of mycobacterium tuberculosis esat6-cfp10 protein on cell viability and production of nitric oxide in alveolar macrophages
conclusions the data indicate that esat6-cfp10 could support the survival of m. tuberculosis in the host through altering the host immune response. it also indicates that the host may gain some level of protection from a second exposure to m. tuberculosis, as evidenced by increased proliferation of nr8383-ec. background mycobacterium tuberculosis is the major pathogen of tuberculosis, which aff...
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ژورنال
عنوان ژورنال: International Journal of Biological Macromolecules
سال: 2021
ISSN: 0141-8130
DOI: 10.1016/j.ijbiomac.2020.12.179